Coding

Part:BBa_K3715005:Design

Designed by: Yuan Wang   Group: iGEM21_TJUSLS_China   (2021-09-25)


Super5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 783
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 783
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 783
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 783
    Illegal NgoMIV site found at 58
    Illegal NgoMIV site found at 112
    Illegal NgoMIV site found at 139
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to improve the stability of the enzyme, we engineered PETase through multiple site mutations.



Source

We make multiple site mutations on the basis of WT（PETase）which is from the PET-consuming microbeIdeonella sakaiensis.

References